| 质粒名称: | pBabe-puro largeTcDNA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 14088 |
| 存储实验室: | William Hahn |
|
载体骨架基本信息 |
|
| 载体名称: | pBabe-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5100 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Large T antigen,Large T cDNA,pBNT,LTc |
| 物种来源: | simian virus |
| 插入大小: | 2200 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI（未销毁） |
| 3' 克隆位点: | BamHI（未销毁） |
| 5' 测序引物 | pBabe 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBabe-puro largeTcDNA was a gift from William Hahn (Addgene plasmid # 14088 ; http://n2t.net/addgene:14088 ; RRID:Addgene_14088) |
| 参考文献部分: | Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM, Hahn WC. Cancer Cell. 2003 May . 3(5):483-95. 10.1016/S1535-6108(03)00088-6 PubMed 12781366 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBabe-puro largeTcDNA 7273 bp ds-DNA circular SYN 19-SEP-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBabe-puro largeTcDNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7273)
AUTHORS Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM,
Hahn WC
TITLE Human mammary epithelial cell transformation through the activation
of phosphatidylinositol 3-kinase.
JOURNAL Cancer Cell. 2003 May . 3(5):483-95.
PUBMED 12781366
REFERENCE 2 (bases 1 to 7273)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 19, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7273
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 16..485
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 550..749
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 950..1366
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1296..1318
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1334..1350
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1404..3530
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
primer_bind complement(3611..3631)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3616..3945
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3796..3931
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3858..3877
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3955..4554
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3955..3974)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4451..4471
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 4775..5244
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5374..5391)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5545..6133)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5625..5644)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6293..7153)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6916..6935
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7154..7258)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 agtgccacct gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
61 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
121 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
181 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
241 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
301 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
361 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
421 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
481 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
541 ccgggaggca agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac
601 tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg
661 tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt
721 tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc
781 aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
841 tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt
901 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
961 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
1021 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
1081 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
1141 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
1201 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
1261 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
1321 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccgcg
1381 accggtcgac cagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
1441 taatggacct tctaggtctt gaaaggagtg cctgggggaa tattcctctg atgagaaagg
1501 catatttaaa aaaatgcaag gagtttcatc ctgataaagg aggagatgaa gaaaaaatga
1561 agaaaatgaa tactctgtac aagaaaatgg aagatggagt aaaatatgct catcaacctg
1621 actttggagg cttctgggat gcaactgaga ttccaaccta tggaactgat gaatgggagc
1681 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
1741 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
1801 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
1861 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
1921 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
1981 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
2041 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
2101 atagtgcctt gactagagat ccattttctg ttattgagga aagtttgcca ggtgggttaa
2161 aggagcatga ttttaatcca gaagaagcag aggaaactaa acaagtgtcc tggaagcttg
2221 taacagagta tgcaatggaa acaaaatgtg atgatgtgtt gttattgctt gggatgtact
2281 tggaatttca gtacagtttt gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc
2341 actataagta ccatgaaaag cattatgcaa atgctgctat atttgctgac agcaaaaacc
2401 aaaaaaccat atgccaacag gctgttgata ctgttttagc taaaaagcgg gttgatagcc
2461 tacaattaac tagagaacaa atgttaacaa acagatttaa tgatcttttg gataggatgg
2521 atataatgtt tggttctaca ggctctgctg acatagaaga atggatggct ggagttgctt
2581 ggctacactg tttgttgccc aaaatggatt cagtggtgta tgacttttta aaatgcatgg
2641 tgtacaacat tcctaaaaaa agatactggc tgtttaaagg accaattgat agtggtaaaa
2701 ctacattagc agctgctttg cttgaattat gtggggggaa agctttaaat gttaatttgc
2761 ccttggacag gctgaacttt gagctaggag tagctattga ccagttttta gtagtttttg
2821 aggatgtaaa gggcactgga ggggagtcca gagatttgcc ttcaggtcag ggaattaata
2881 acctggacaa tttaagggat tatttggatg gcagtgttaa ggtaaactta gaaaagaaac
2941 acctaaataa aagaactcaa atatttcccc ctggaatagt caccatgaat gagtacagtg
3001 tgcctaaaac actgcaggcc agatttgtaa aacaaataga ttttaggccc aaagattatt
3061 taaagcattg cctggaacgc agtgagtttt tgttagaaaa gagaataatt caaagtggca
3121 ttgctttgct tcttatgtta atttggtaca gacctgtggc tgagtttgct caaagtattc
3181 agagcagaat tgtggagtgg aaagagagat tggacaaaga gtttagtttg tcagtgtatc
3241 aaaaaatgaa gtttaatgtg gctatgggaa ttggagtttt agattggcta agaaacagtg
3301 atgatgatga tgaagacagc caggaaaatg ctgataaaaa tgaagatggt ggggagaaga
3361 acatggaaga ctcagggcat gaaacaggca ttgattcaca gtcccaaggc tcatttcagg
3421 cccctcagtc ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt
3481 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
3541 attgttgttg ttaacgggga tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg
3601 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
3661 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3781 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3841 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
3901 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc
3961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
4021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
4081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
4141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
4201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
4261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
4321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
4381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
4441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
4501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
4561 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataaaataa
4621 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
4681 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
4741 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
4801 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
4861 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
4921 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
4981 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
5041 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
5101 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
5161 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
5221 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
5281 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
5341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
5401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
5461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
5521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
5581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
6001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
6061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
6121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
6181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
6241 tttgcggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
6301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
6361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
6421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
6481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
6541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
6601 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
6661 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
6721 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
6781 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
6841 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
6901 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
6961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
7021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
7081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
7141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
7201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
7261 catttccccg aaa
//
